OTS mRNA - FLuc

FLuc mRNA is ready to use and contains no detectable protein or dsRNA. Our FLuc mRNA harbours several mutations (protein level) that enhance the folding and increase the stability of the protein, so you can detect even the lowest of protein expression levels.

In addition, our FLuc mRNA has optimized, proprietary RIBOPRO 5’- and 3’- UTRs, >95% capping (Cap1), and has extraordinary low levels of dsRNA, ensuring high activity and low toxicity. It is provided at 1 mg/mL in RNAse-free water

Best choice for:
In vivo bio-distribution experiments

Frequently used for:
Localization of transfected cells, positive control

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Fast delivery
Great to quantify performance
Direct available for order

FLuc Product Specifications

Our FLuc mRNA expresses the firefly luciferase protein. In the presence of its substrate, Luciferin, FLuc mRNA translates into bioluminescent FLuc. FLuc is widely used as a fluorescence reporter gene and is commonly used in biodistribution studies.

Protein Characteristics:

Attribute	Specification
Luminescence Color	Yellow-Green luminescense
Emission Maximum (λmax)	550 nm
Mutations	N50D, N119G, T214A, A215L, I232A, F295L, E354K, D404N, C-terminal SKL->IAV

mRNA Specifications:

Attribute	Specification
Length (total)	1816 nt (incl. 125 nt A-tail)
Cap Structure	Cap-1; standard capping, 95%+ capping efficiency
Poly(A) Tail	125 nt
5′-UTR	High activity
3′-UTR	Normal stability
dsRNA/DNA	Reduced
Purification	Supramagnetic beads
Labeling	None

Analytical Properties:

Attribute	Specification
Molecular Weight	588,048.4 g/mol
GC Content	43.9%
fmol/ng	1.70

Available Customizations:

Category	Options
Buffer	PBS (pH 7.4), Citrate (pH 5.5), Other
Additional QC	dsRNA ELISA, Endotoxin Testing, Sanger Sequencing, Transfection Efficiency Test (HeLa cells)
Modified Nucleotides	N1-methylpseudouridine, 5-methylcytosine
Labeling	AF647, AF568, AF488, other dyes available upon request
Scale	Standard scales from 100 µg to 1 mg direct from stock, or custom scales from 1 mg

FLuc Applications

Firefly Luciferase (FLuc) mRNA is a highly sensitive reporter commonly used to evaluate mRNA delivery, transfection efficiency, and in vivo protein expression. The data demonstrate representative results obtained using RiboPro’s FLuc mRNA in both animal and cell-based transfection studies.

Bioluminescence imaging performed 6 hours after intravenous administration of FLuc mRNA encapsulated in lipid nanoparticles (LNPs) showed robust luciferase expression in C57BL/6 mice. Following injection of 0.5 mg/kg FLuc mRNA, strong bioluminescent signals were detected, demonstrating efficient in vivo delivery and protein expression.

In vitro transfection studies in bone marrow-derived dendritic cells (BMDCs) further confirmed the performance of FLuc mRNA. Luciferase activity was measured after 24 and 48 hours following delivery using PLGA/PBAE nanoparticles, PBAE nanoparticles, or Lipofectamine 2000. All delivery systems mediated detectable expression, with Lipofectamine 2000 producing the highest luciferase activity, while PLGA/PBAE formulations showed substantial reporter expression compared to untreated controls.

Characteristics:

Specification	Value
Concentration	1 µg/µl
Storage Buffer	RNase-free water
mRNA length	1812 nt
Handling & Storage	Short-term storage at -20°C; long-term storage at -80°C

Further details on the experimental setup and results can be found in the corresponding publication:

1) Bioluminescence imaging data adapted from published in vivo FLuc mRNA delivery studies.

2) BMDC transfection data adapted from published FLuc mRNA nanoparticle delivery studies.

How to get a quote?

By filling in our ‘Request Quote’ form, you can fill in your choice and volume of mRNA. After submitting, we will contact you with a quote as soon as possible. In the form you can also add questions, remarks and ask our Sales Specialists anything that you want to know. Filling out the form is fast, easy, and prepares our people to support you the best they can!

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Head of RNA Operations
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Real-world evidence

What our customers say

“I really value the helpful contact with the Product Specialists that answered all my questions. This resulted in rapid and excellent service form RiboPro.”

Dr. Piter Bosma

PI at Amsterdam UMC

“We are RiboPro customer since 2020 and we are satisfied about their products. We also appreciate very much the customer service. The team is very cooperative and helpful, communication is clear, precise and always on time.”

Chiara Gai

"The staff at RiboPro have been very friendly and helpful throughout the ordering process."

Ben Wylie

“RiboPro provided both the expert support and synthesized the high-quality mRNA to make our first gene manipulation experiments a resounding success. Furthermore, RiboPro was able to provide the mRNA constructs at an inordinate speed.”

Aviad Pato

Head of research at Gamida Cell

“Good service paired with quick delivery and competitive pricing. Quality of delivered material was also very good. I can recommend RiboPro as a supplier of mRNA material.”

Arturs Aksjonovs

“We have been ordering RNA from RiboPro, and find that they produce very good quantity product. Also, their customer service and technical support are excellent! Highly recommend this company.”

Albert Kwok

"Highly recommend Ribopro. Communication was fast and clear. They offer custom mRNA products at a reasonable price."

Eu Ddeum Chung

“RiboPro offers not only high-quality mRNA but also very helpful and fast support on all matters concerning mRNA.”

Jana Schembera

Researcher

“Initially my results with mRNA were suboptimal, however with the support and dedication from the professionals at RiboPro, I was able to further optimize my experiments in such a way that my experiments turned out more successful.”

Jitske Jansen

Post-doc researcher Radboudumc

"High quality reagents, clear communication and overall great service."

Joy Seiler

“I ordered a custom Cap-1 mRNA from RiboPro for in vivo research. The custom mRNA from RiboPro expressed as the target protein in the mammalian cell line and maintained good level of protein expression in vivo (mouse). They delivered me the mRNA product in a timely manner and gave useful comments, responses and transparency to my questions for my order. So, I would recommend users trying the RiboPro.”

Lee Jenny

“The high quality, stable and non-immunogenic mRNA generated by Ribopro is extremely suitable to meet our needs. The company also offers the qualified expert advice allowing the optimization of our experimental protocols.”

Prof. Dr. Elena Levtchenko

Professor at KU Leuven